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KMID : 1189420170300040049
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2017 Volume.30 No. 4 p.49 ~ p.61
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Skin Whitening Effect of Leonuri Herba Extracts(LHE) on Malignant Melanoma Cell
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Kim Ji-Eun
Lim Kyeong-Min
Na Ga-Young
Kim Hye-Hwa
Kang Byung-Soo
Choi Jeong-Hwa
Park Su-Yeon
Jung Min-Young
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Abstract
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Objectives : In order to find out the whitening effects of Leonuri Herba, this study was designed to identify the effects and the action mechanism of LHE(Leonuri Herba extract) on Malignant melanoma cell lines.
Methods : After treating LHE on the B16F10 cell-Malignant melanoma cell line-, the cell survival rate, melanin biosynthesis rate, intra&extracellular tyrosinase activity rate, SOD-like activity, tyrosinase mRNA gene expression were investigated. The results were compared with control group without LHE treatment or with positive control group treated with whitening efficacy substance.
Results : B16F10 cell survival rate, melanin biosynthesis rate, and intra&extracellular tyrosinase activity were significantly inhibited depending on the concentration of treated LHE. Melanin biosynthesis rate and tyrosinase activity rate were also decreased when α-MSH was combined with LHE. In addition, the SOD-like activity was increased in a concentration-dependent manner in the treatment with the LHE, indicating signigicant activity at high concentrations, and the tyrosinase mRNA gene expression was decreased in both the LHE-treated group, the LHE and α-MSH-treated group.
Conclusions : LHE seems to inhibit melanin synthesis through inhibition of tyrosinase activity and inhibition of tyrosinase mRNA gene expression. It also has the effect of promoting SOD-like activity and may be used clinically as a skin whitening agent in the future.
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KEYWORD
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Leonuri Herba(LH) , skin whitening , malignant melanoma cell
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